Rapid presumptive diagnosis clostridial infections by gas chromatography.
نویسندگان
چکیده
منابع مشابه
Rapid diagnosis of anaerobic infections by gas-liquid chromatography.
It was postulated that the short chain fatty acids (SCFA) produced by anaerobic bacteria might serve as microbial markers in purulent material. Eighteen pus specimens from various sources were analysed by gas-liquid chromatography (GLC), and the SCFA detected were compared with the microorganisms isolated by conventional methods. It was found that the detection of propionic, isobutyric, butyric...
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Gas-liquid chromatographic analysis of volatile fatty acid patterns was performed when growth was initially detected in 87 positive blood cultures. Isovaleric acid, butyric acid, or both were found in 39 of 43 (90%) blood cultures that contained anaerobes but were absent in all 44 cultures that contained facultatively anaerobic or aerobic bacteria. The detection of isovaleric acid indicated the...
متن کاملRapid diagnosis of anaerobic infections by direct gas-liquid chromatography of clinical speciments.
Current methods to isolate and identify anaerobic bacteria are laborious and time consuming. It was postulated that the short-chain fatty acids (SCFA) produced by these organisms might serve as microbial markers in clinical material. 98 specimens of pus or serous fluid were analyzed by gas-liquid chromatography, and findings were compared with culture results. Good correlations were found for t...
متن کاملPresumptive diagnosis of anaerobic bacteremia by gas-liquid chromatography of blood cultures.
By quantitative gas-liquid chromatography of glucose-containing blood cultures at the moment of first signs of growth, a presumptive diagnosis of anaerobic bacteremia could be made in 24 out of 26 cultures yielding obligate anaerobes upon subsequent culture. With Bacteroides sp. (20 strains isolated), elevated levels of isovaleric acid (greater than or equal to 0.1 mumol/ml) and/or succinic aci...
متن کاملGas liquid chromatography in the rapid diagnosis of meningitis.
Gas liquid chromatography was employed to detect lactic acid in the cerebrospinal fluid of patients suspected of having bacterial meningitis. The technique was found to be both rapid and reliable. Differentiation between aseptic, purulent, and partially treated bacterial meningitis was readily achieved. The effectiveness of therapy in bacterial meningitis was indicated by a rapid fall in the co...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1987
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.49.1053